ruminococcus bromii atcc 27255 Search Results


r bromii atcc 27255 t  (ATCC)
95
ATCC r bromii atcc 27255 t
R Bromii Atcc 27255 T, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ruminococcus bromii  (ATCC)
95
ATCC ruminococcus bromii
(A) Gating strategy for identification of ILC3s via flow cytometry. ILC3s were defined as CD45+Viable Lymphocyte Single Cells that were lineage- CD127+ and CD117+. (B) Flow plots from a representative sample illustrating granzyme B expression in ILC3s after LPMCs were exposed to <t>R.bromii</t> (Rb), A.junii (Aj), or S. typhimurium (St) . Isotype controls were used to establish positive granzyme B staining. (C) Percentages of ILC3s expressing granzyme B after LPMC exposure to bacteria. N=5. Statistical analysis was one-way ANOVA. *p<0.05, n.s. not significant.
Ruminococcus Bromii, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ruminococcus+bromii+atcc+27255/Ruminococcus+bromii+Moore+et+al/pmc08664910-43-0-2
Average 95 stars, based on 1 article reviews
ruminococcus bromii - by Bioz Stars, 2026-10
95/100 stars
  Buy from Supplier

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(A) Gating strategy for identification of ILC3s via flow cytometry. ILC3s were defined as CD45+Viable Lymphocyte Single Cells that were lineage- CD127+ and CD117+. (B) Flow plots from a representative sample illustrating granzyme B expression in ILC3s after LPMCs were exposed to R.bromii (Rb), A.junii (Aj), or S. typhimurium (St) . Isotype controls were used to establish positive granzyme B staining. (C) Percentages of ILC3s expressing granzyme B after LPMC exposure to bacteria. N=5. Statistical analysis was one-way ANOVA. *p<0.05, n.s. not significant.

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: Gut bacteria induce granzyme B expression in human colonic ILC3s in vitro in an IL-15-dependent manner

doi: 10.4049/jimmunol.2000239

Figure Lengend Snippet: (A) Gating strategy for identification of ILC3s via flow cytometry. ILC3s were defined as CD45+Viable Lymphocyte Single Cells that were lineage- CD127+ and CD117+. (B) Flow plots from a representative sample illustrating granzyme B expression in ILC3s after LPMCs were exposed to R.bromii (Rb), A.junii (Aj), or S. typhimurium (St) . Isotype controls were used to establish positive granzyme B staining. (C) Percentages of ILC3s expressing granzyme B after LPMC exposure to bacteria. N=5. Statistical analysis was one-way ANOVA. *p<0.05, n.s. not significant.

Article Snippet: Ruminococcus bromii (ATCC# 27255) was grown in liquid chopped meat broth (Hardy Diagnostics) for 1–2 days under anaerobic conditions at 37°C using the BD GasPak EZ Anaerobe Pouch System according to manufacturer’s instructions (BD Diagnostics, Franklin Lakes, NJ).

Techniques: Bacteria, Expressing, Flow Cytometry, Staining

(A) Flow plots from a representative sample illustrating perforin expression in ILC3s after LPMCs were exposed to R.bromii (Rb), A.junii (Aj), or S. typhimurium (St) . Isotype controls were used to establish positive perforin staining. (B) Percentages of ILC3s expressing perforin, (C) granzyme B and perforin, or (D) granzyme B and not perforin after LPMC exposure to bacteria. N=5.

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: Gut bacteria induce granzyme B expression in human colonic ILC3s in vitro in an IL-15-dependent manner

doi: 10.4049/jimmunol.2000239

Figure Lengend Snippet: (A) Flow plots from a representative sample illustrating perforin expression in ILC3s after LPMCs were exposed to R.bromii (Rb), A.junii (Aj), or S. typhimurium (St) . Isotype controls were used to establish positive perforin staining. (B) Percentages of ILC3s expressing perforin, (C) granzyme B and perforin, or (D) granzyme B and not perforin after LPMC exposure to bacteria. N=5.

Article Snippet: Ruminococcus bromii (ATCC# 27255) was grown in liquid chopped meat broth (Hardy Diagnostics) for 1–2 days under anaerobic conditions at 37°C using the BD GasPak EZ Anaerobe Pouch System according to manufacturer’s instructions (BD Diagnostics, Franklin Lakes, NJ).

Techniques: Expressing, Staining, Bacteria

(A, C, F) Flow plots from a representative sample illustrating (A) IL-22, (C) IL-22 and granzyme B or (F) IL-22 and perforin expression in ILC3s after LPMCs were exposed to R.bromii (Rb), A.junii (Aj), or S. typhimurium (St) . Isotype controls were used to establish positive IL-22, granzyme B and perforin staining. (B) Percentages of ILC3s expressing IL-22, (D) co-expressing granzyme B and IL-22, (E) expressing granzyme B only and not IL-22, (G) co-expressing perforin and IL-22 or (H) expressing perforin only and not IL-22, after LPMC exposure to bacteria R.bromii (Rb), A.junii (Aj), or S. typhimurium (St) . N=5. Statistical analysis was one-way ANOVA. *p<0.05, n.s. not significant.

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: Gut bacteria induce granzyme B expression in human colonic ILC3s in vitro in an IL-15-dependent manner

doi: 10.4049/jimmunol.2000239

Figure Lengend Snippet: (A, C, F) Flow plots from a representative sample illustrating (A) IL-22, (C) IL-22 and granzyme B or (F) IL-22 and perforin expression in ILC3s after LPMCs were exposed to R.bromii (Rb), A.junii (Aj), or S. typhimurium (St) . Isotype controls were used to establish positive IL-22, granzyme B and perforin staining. (B) Percentages of ILC3s expressing IL-22, (D) co-expressing granzyme B and IL-22, (E) expressing granzyme B only and not IL-22, (G) co-expressing perforin and IL-22 or (H) expressing perforin only and not IL-22, after LPMC exposure to bacteria R.bromii (Rb), A.junii (Aj), or S. typhimurium (St) . N=5. Statistical analysis was one-way ANOVA. *p<0.05, n.s. not significant.

Article Snippet: Ruminococcus bromii (ATCC# 27255) was grown in liquid chopped meat broth (Hardy Diagnostics) for 1–2 days under anaerobic conditions at 37°C using the BD GasPak EZ Anaerobe Pouch System according to manufacturer’s instructions (BD Diagnostics, Franklin Lakes, NJ).

Techniques: Expressing, Staining, Bacteria

(A) Flow plots from a representative sample illustrating granzyme B expression in ILC3s after LPMCs were exposed to S. typhimurium or recombinant IL-15 alone (IL-15) or in combination with (IL-15+IL-12) or IL-2 (IL-15+IL-2). Isotype controls were used to establish positive granzyme B staining. (B) Percentages of granzyme B expression in ILC3s after LPMCs were exposed to IL-15, IL-15+IL-2, IL-15+IL-12p70, or S. typhimurium . N=4. (C) Measurement of IL-15 (pg/mL) in cell supernatant after LPMC exposure to bacteria R.bromii (Rb), A.junii (Aj), or S. typhimurium (St) . N=5. (D) Percentages of ILC3s expressing granzyme B after LPMC exposure to A. junii in the presence of antibody targeting IL-15 or control IgG antibody. N=5. (E) Percentages of ILC3s expressing granzyme B after LPMC exposure to S. typhimurium in the presence of antibody targeting IL-15 or control IgG antibody. N=4. Statistical analysis was one-way ANOVA or paired t test. **p<0.01, *p<0.05, n.s. not significant.

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: Gut bacteria induce granzyme B expression in human colonic ILC3s in vitro in an IL-15-dependent manner

doi: 10.4049/jimmunol.2000239

Figure Lengend Snippet: (A) Flow plots from a representative sample illustrating granzyme B expression in ILC3s after LPMCs were exposed to S. typhimurium or recombinant IL-15 alone (IL-15) or in combination with (IL-15+IL-12) or IL-2 (IL-15+IL-2). Isotype controls were used to establish positive granzyme B staining. (B) Percentages of granzyme B expression in ILC3s after LPMCs were exposed to IL-15, IL-15+IL-2, IL-15+IL-12p70, or S. typhimurium . N=4. (C) Measurement of IL-15 (pg/mL) in cell supernatant after LPMC exposure to bacteria R.bromii (Rb), A.junii (Aj), or S. typhimurium (St) . N=5. (D) Percentages of ILC3s expressing granzyme B after LPMC exposure to A. junii in the presence of antibody targeting IL-15 or control IgG antibody. N=5. (E) Percentages of ILC3s expressing granzyme B after LPMC exposure to S. typhimurium in the presence of antibody targeting IL-15 or control IgG antibody. N=4. Statistical analysis was one-way ANOVA or paired t test. **p<0.01, *p<0.05, n.s. not significant.

Article Snippet: Ruminococcus bromii (ATCC# 27255) was grown in liquid chopped meat broth (Hardy Diagnostics) for 1–2 days under anaerobic conditions at 37°C using the BD GasPak EZ Anaerobe Pouch System according to manufacturer’s instructions (BD Diagnostics, Franklin Lakes, NJ).

Techniques: Bacteria, Expressing, Recombinant, Staining, Control